Web提供昆虫细胞色素P450与抗药性关系研究进展_杨帆文档免费下载,摘要:四川动物2008年第27卷第3期SichuanJournalofZoologyVol27No32008 ... WebDegenerate primers corresponding to conserved amino acid sequences in three insect P450s, Musca domestica (CYP6A1), Drosophila melanogaster (CYP6A2) and Papilio polyxenes (CYP6B1), were used to clone xanthotoxin-induced P450 transcripts from P. glaucus larvae by a reverse transcription-polymerase chain reaction (RT-PCR) strategy.
Point mutations associated with insecticide resistance in the ...
WebJan 1, 2016 · Our research indicated that CYP6A2 was the only up-regulated P450 gene among the differentially expressed genes (DEGs) in the spirotetramat-resistant strain via … Webthe Cyp6a8 and Cyp6a2 genes, respectively, has identified the presence of several putative AP1 binding motifs (Bhaskara et al., 2006). It is possible that caffeine induction of Cyp6a2 and Cyp6a8 genes may be mediated via the signaling pathways described above and the putative AP1 binding motifs. To test these possibilities and to incassobureau westland
昆虫细胞色素P450与抗药性关系的分子生物学研究进展
WebOnly a handful of P450 genes have been functionally characterized from the approximately 90 recently identified in the genome of Drosophila melanogaster. Cyp6a8 encodes a 506 … The psiCHECK-2 dual fluorescent reporter system (Promega, Madison, WI, USA) was used to determine any interaction of miR-310s with putative target binding sites in Cyp6g1 and Cyp6g2. The psiCHECK-2 system incorporates Renilla luciferase as a reporter gene and firefly luciferase as a control to normalize for … See more The DDT-susceptible Canton-S, the low-level DDT-resistant 91-C, and the highly DDT-resistant 91-R strains of Drosophila have been … See more First-strand cDNA synthesis was performed using the miScript II RT kit (Qiagen). Each synthesized cDNA sample was then used as a template for RT-qPCR reactions using the miScript SYBR Green PCR Kit (Qiagen) … See more A set of mirVana mimics were synthesized for miR-310, miR-311, miR-312, and miR-313 by Invitrogen (Ambion, Life Technologies) at a … See more One-day-old female 91-R (90 flies per each injection) were injected separately with miR-310s mimics, NCsiRNA, and DEPC-water using … See more WebMay 1, 2000 · Europe PMC is an archive of life sciences journal literature. This website requires cookies, and the limited processing of your personal data in order to function. inclusive writing in french