Immortalized human hepatocytes

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August undefined, 2024

Witryna19 gru 2013 · Cell lines: Immortalized Human Hepatocytes (IHH), HepG2, Hep3B (hepatoma cell lines) and HeLa-S3 (immortal cell line derived from cervical cancer cells) were cultured in DMEM (Dulbecco modified Eagle) medium glucose containing 4.5 g/L of glucose and supplemented with 10% fetal calf serum (Invitrogen, Saint Aubin, … WitrynaAn immortalized human hepatocyte cell line, HepLi5, was established via transfection of Simian virus 40 large T antigen (SV40 LT) into primary human hepatocytes. The …

Cannabinoid receptor 1 and 2 agonists increase lipid accumulation …

WitrynaResults: The immortalized hepatocytes grew continuously yet were non-tumorigenic, stopped proliferating when exposed to Cre recombinase, and conserved defining properties of primary hepatocytes, including the ability to secrete liver-specific proteins and to detoxify drugs. Witryna13 kwi 2024 · Functional human hepatocytes as surrogates for PHH have been generated in vitro using stem cell-based techniques, ... HNF4A, and HNF1A … porting telephone numbers to microsoft teams

Long-term culture and characterization of patient-derived primary ...

Witryna13 kwi 2024 · In vitro-in vivo extrapolation ((IVIVE) and empirical scaling factors (SF) of human intrinsic clearance (CL int) were developed using one of the largest dataset of 455 compounds with data from human liver microsomes (HLM) and human hepatocytes (HHEP).For extended clearance classification system (ECCS) class 2/4 … WitrynaAbstract. We previously reported that the telomere-targeting drug telomestatin induces apoptosis accompanied by G-tail reduction and dissociation of binding protein TRF2 … Witryna15 lis 2024 · Immortalized human hepatocytes (IHH) could provide an unlimited supply of hepatocytes, but insufficient differentiation and phenotypic instability restrict their clinical application. optical coherence elastography

Establishment of functional telomerase immortalized human …

In vitro Differentiation of Human TERT-Transfected Multi-Lineage ...

Witryna30 wrz 2011 · The development of continuous non-tumorous cell line steadily containing hepatocyte phenotypes would substitute the primary hepatocytes for these studies. … WitrynaImmortalized differentiated liver cell lines capable of continuous proliferation, and expressing stable liver-specific functions, would be valuable for in vitro toxicity testing … optical coherence refraction tomographyWitrynaPrevious research focused on immortalization methods, such as telomerase and viral, to culture immortalized primary human hepatocytes, which may lose some of the normal properties. However, non-immortalized PHHs often fail to maintain long-term viability and functionality. porting teams numbers

"Witryna4 cze 2024 · Human primary hepatocytes (PHs) are critical to studying liver functions, drug metabolism and toxicity. PHs isolated from livers that are unacceptable for transplantation have limited expansion and culture viability in vitro, in addition to rapidly deteriorating enzymatic functions. The unsuitability of immortalized hepato … " - Immortalized human hepatocytes

Immortalized human hepatocytes

Characterization of lipid metabolism in a novel immortalized human ...

Witryna4 cze 2024 · A methodology for the development of immortalized hepatocyte-like hybrid cells by the in vitro fusion of human MLPC with normal human primary hepatocytes is reported. The resultant hybrid cells exhibited homology with hepatocytes by morphology, immunohistochemistry, urea and albumin production and gene expression. WitrynaTo develop a murine model for HBV production, we established an immortalized, cloned liver cell line by transferring the Simian Virus 40 Large T-Antigen into primary human …

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WitrynaAbstract. The lack of a suitable in vitro hepatitis B virus (HBV) infectivity model has limited examination of the early stages of the virus-cell interaction. In this study, we … WitrynaHere we report the immortalization of human hepatocytes by the introduction of HPV16 E6/E7 and hTERT. Human adult hepatocytes were lentivirally transduced with …

WitrynaImmortalized Human Hepatocytes for In Vitro Research and Drug Development. Immortalized Fa2N-4 hepatocytes allow you to compare results using hepatocytes … WitrynaFor in vitro study, Hc3716 cells, immortalized human hepatocytes, were treated with fatty acids in the presence or absence of LX2, immortalized HSCs. Results: Choline-deficient l-amino acid-defined high-fat diet increased pronounced hepatic steatosis, which was attenuated by GTX treatment, together with a reduction in the number of activated ...

Witryna8 lut 2015 · This study aimed to establish immortalized human HSC using the simian virus 40 large T antigen (SV40LT) for applications in co-culturing with hepatocytes … Witryna1 sty 2015 · 1. Introduction. At present, primary human hepatocytes represent an important tool for research purposes, in particular in the field of in vitro pharmaco-toxicology ().However, their use is largely impeded by inadequate supply, high cost, high variability and limited in vitro proliferation capacity. Alternative cell sources include …

WitrynaA breakthrough in cell biology research Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology research that combines the in vivo nature of primary cells with the traditional cell line's ability to survive continuously in vitro.

Witryna15 gru 2012 · Aside from genetically modified liver cell lines and immortalized human hepatocytes, other functional hepatocytes derived from stem cells show great … porting templateWitrynaPrimary human hepatocytes were immortalized using lentiviral vectors coding for antiapoptotic genes and microencapsulated using alginate-polylysine polymers. In … optical coherence tomography applicationWitrynaTarget mRNAs and pathways were predicted for the miRNAs most significantly upregulated in NAFLD, their cell-type-specific expression was investigated by … porting the gnu debuggerWitryna15 wrz 2015 · We sought to develop and characterize a novel human liver cell line (LIV0APOLY) to establish a model of lipid accumulation using a physiological mixture of fatty acids under low- and high-glucose conditions. LIV0APOLY cells were compared with a well-established cell line (HepG2) and, where possible, primary human hepatocytes. porting testWitrynaMethods: We used an in vitro model of fatty liver by treating immortalized human hepatocytes and HepG2 cells with oleic acid and the selective agonists arachidonyl-2 … optical coherence tomography codeWitrynaImmortalized hepatocytes (Fa2N-4 cells) have been proposed as a tool to identify CYP3A4 inducers. The purpose of the current studies was to characterize the effect of … porting the numberWitrynaTwo human immortalized hepatocyte cell lines were established and were named HHE6E7T-1 and HHE6E7T-2. Those cells proliferated in culture beyond 200 … optical coherence tomography indications