P lvsh rna-egfp 2a puro

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August undefined, 2024

WebThe pLVshRNA promotor is combined with a EGFP (2A)Puro vector sequence and a INSM1 gene in the plasmid vector map. Conjugate The Green Fluorescent Protein (eGFP) of … WebWe found a significant difference between the follicular FSH levels in normal oocytes and abnormal oocytes (4.85 ± 1.75 IU/L vs. 5.41 ± 2.47 IU/L, respectively; p = 0.021). We found …

pLenti-U6-shRNA-(GFP-Puro) Cloning Kit - GenTarget Inc

WebDec 28, 2024 · pLVshRNA-EGFP(2A) Puro plasmid is a lentiviral RNA interference vector based on HIV-1, which is used to express the shRNA synthesized artificially and interfere … WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. cvs pharmacy in richardson

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WebpLVshRNA-EGFP (2A)Puro载体是基于HIV1的慢病毒RNA干扰载体，用于表达人工设计合成的shRNA，干扰目标基因的表达。 RNA干扰序列插入到载体中BamHI和EcoRI之间，由III类RNA聚合酶启动子U6启动子转录产生shRNA，经剪切后产生成熟siRNA，产生干扰效果。 PGK启动子驱动表达EGFP基因和Puromycin抗性基因。可以使用Puromycin筛选出稳定过 … WebApr 14, 2024 · 应用siRNA(small interference RNA)技术敲低FLT1后根据FLT1蛋白表达量筛选出敲低效果最明显的siRNA。分别在转染pLV-EGFP(2A)Puro-KLF8质粒和pLV-EGFP(2A)Puro空载体质粒的769-P细胞中转染siFLT1,再应用平板克隆实验,MTS实验检测769-P细胞株增殖能力的变化。 EGFP-P2A-PuroR Species Synthetic Insert Size (bp) 1383 Promoter LTR Cloning Information Cloning method Gateway Cloning 5′ sequencing primer EGFP-N 3′ sequencing primer Puro-F (Common Sequencing Primers) Resource Information Supplemental Documents JS76 - pMMLV-EGFP-P2A-Puro.gbk A portion of this plasmid was derived from a plasmid made by cheap flights dfw to heathrow

Lenti-AP1-TA-Luc-EF1α-mCherry (10^8TU/ml, 报告基因慢病 …

pLVX-Puro Vector Information - Takara Bio

WebAbstract. A new genetic marker was created in which sequences from enhanced green fluorescent protein were fused to those of puromycin N-acetyl transferase. The resulting … WebOct 1, 2024 · The donor plasmid was modified by adding a ‘2A’ sequence in front of EGFP. Two million hiPSCs were electroporated with 3 μg of the OCT4-targeting gRNA-Cas9 … cvs pharmacy in richardson txWebThe MISSION ® eGFP shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing eGFP. Ampicillin and puromycin antibiotic resistance genes … cheap flights discount code

"WebpLVshRNA-EGFP(2A)Puro-Hdac6-m-shRNA1. PVT20147 2ug. Product Name:pLVshRNA-EGFP(2A)Puro-Hdac6-m-shRNA1 Promoter: U6 Replicon: pUC Prokaryotic resistance: Amp Host: lentiviral plasmid Vector type: gene silencing Fragment type: shRNA species: Mouse Prokaryotic resistance: Amp Resistance Marker: Puro Fluorescent labeling: Green … " - P lvsh rna-egfp 2a puro

P lvsh rna-egfp 2a puro

pLVX-Puro Vector Information - Takara Bio

Web一抗二抗内参抗体标签抗体抗原植物抗体病毒抗体细菌抗体小分子抗体 RNA ... 当前位置: 首页 > 分子生物学 > 重组克隆表达 > 质粒 > pLVshRNA-EGFP(2A)Puro-DLGAP5-shRNA1. pLVshRNA-EGFP(2A)Puro-DLGAP5-shRNA1 VT009291说明书 ... WebJun 30, 2015 · The human HPRT targeting vector pHPRT-LH 2A-EGFP-2A-Puro or pHPRT-SH 2A-EGFP-2A-Puro was transfected into HT1080 cells, and puromycin-resistant colonies were counted to calculate the total integration frequency. Subsequently, single colonies were isolated and expanded to prepare genomic DNA.

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WebpLVshRNA-EGFP (2A)Puro-FKBP1A (human)-shRNA1 Description Promoter:U6 Replicator:pUC Clone strain:Stbl3 Culture conditions:37centigrade Host:mammalian … WebDec 30, 2015 · The viral 2A sequence is suitable for gene manipulation in the Siberian hamster. • It allows long-term simultaneous over-expression of 2 genes in vitro and in vivo. • We demonstrate dual expression in vitro in the neuroblastoma cell line SH-SY5Y. • We demonstrate dual expression in the hypothalami of Siberian hamsters and mice. Abstract

WebFamily: CRISPR sgRNA Constructs, Vectors, and Cells Catalog #: SVCRU616-L Standard sgRNA U6 Promoter Expression Vector (linearized, ready-for-cloning) $600.00 U.S. List Price more info Prices shown reflect U.S. list pricing for orders shipped to U.S. addresses. Non-U.S. pricing may vary depending on geography. WebeGFP shRNA (SHC005, SHC005V) The eGFP shRNA vector consists of the TRC1.5-pLKO.1–puro vector, containing shRNA that targets eGFP (GenBank Accession No. pEGFP U55761), and can be used as a positive control to quickly visualize knockdown.

Web1.A nucleolytically inactive Cas9-VP64 fusion: dCas9 is used to ensure that no strand breaks are introduced into endogenous genome; VP64 is a transcription activation domain that acts synergistically with p65 and HSF1 to enhance transcription. WebpLVshRNA-EGFP (2A)Puro-FKBP1A (human)-shRNA1 Description Promoter:U6 Replicator:pUC Clone strain:Stbl3 Culture conditions:37centigrade Host:mammalian cells,Lentivirus Use:gene silencing Species:Human Gene type:shRNA Prokaryotic resistance:Amp Eukaryotic resistance:Puro Fluorescent protein:Green Caution: 1. This …

WebpLenti-U6-shRNA- (GFP-Puro) Cloning Kit is designed to cloning double-strand DNA encoding a short hairpin RNA. Once transcribed, the shRNA was processed into short RNA …

WebpLL-CMV-GFP-T2A-Puro Lenti-Labeler™ Lentivector Plasmid & Pre-packaged Virus Reliably label the cells of your choice with copGFP driven by the strong CMV promoter (and with … cheap flights domestic indiaWebProduct: 2A peptide from Thosea asigna virus capsid protein Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. T2A 5534 .. 5587 = 54 bp 18 amino acids = 1.8 kDa Product: … cvs pharmacy in richland waWebMar 31, 2024 · P values were calculated by a two-way ANOVA with Sidak’s multiple comparisons test. ****P < 0.0001. b The hPolI-PeV-A3-EGFP and mPolI-PeV-A3-EGFP plasmids contain human (hPolI) and mouse (mPolI) RNA polymerase I promoters in addition to PeV-A3-EGFP DNA, respectively, and also have a transcription terminator DNA (mTer) … cheap flights domestic jetstarWeb慢病毒载体pLV-EGFP（2A)puro（货号：VL3402）包装慢病毒后感染SMMC‐7721细胞，用嘌呤霉素筛选得到EGFP过表达多克隆细胞株。收到细胞的处理：根据客户所在地及发货季节，我们可能采用干冰运输的冻存细胞或者常温运输的培养瓶细胞两种不同形式发货。 cheap flights dohopWebMay 7, 2024 · RNA Control 1, SKU:102024 ) dissolved in either ddH 2 O or in a 50:50 ddH 2 O:Quick Extract mixture. We set up each RT-qPCR reaction with a total volume of 10 µl (1 … cheap flights domestic australiaWebpLenti-CMV-GFP-2A-Puro-Blank Vector from Applied Biological Materials (ABM). Cat Number: LV591. UK & Europe Distribution. Order Online. Toggle menu. Topsan Gentaur Genprice Group; Marienbongard 20 52062 Aachen Deutschland; ... abm rna & cdna controls. €182.00 abm ORF1ab and S Gene 21194 – 22194 Fragment cDNA Control G633-4Insert … cvs pharmacy in richmond hts ohioWebConclusion The recombinant plasmid pLV-EGFP (2A)Puro-SEPP1 has been constructed successfully. Overexpression of SEPP1 could significantly reduce the proliferation rate of 786-O and 769P cells, and cause G2/M phase arrest of 786-O cells. MeSH terms Blotting, Western Carcinoma, Renal Cell / genetics Carcinoma, Renal Cell / metabolism cheap flights doha to manila